Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA
Identifieur interne : 000D35 ( Main/Exploration ); précédent : 000D34; suivant : 000D36Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA
Auteurs : Amir H. Ghassemi [Pays-Bas] ; Mies J. Van Steenbergen [Pays-Bas] ; Herre Talsma [Pays-Bas] ; Cornelus F. Van Nostrum [Pays-Bas] ; Daan J. A. Crommelin [Pays-Bas] ; Wim E. Hennink [Pays-Bas]Source :
- Pharmaceutical Research [ 0724-8741 ] ; 2010.
Abstract
To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA).
BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on
Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5–10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from ~20 days to 60 days when the HMG content decreased from 50% to 25%.
Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres.
Url:
DOI: 10.1007/s11095-010-0205-8
PubMed: 20602152
PubMed Central: 2916118
Affiliations:
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Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Van Steenbergen, Mies J" sort="Van Steenbergen, Mies J" uniqKey="Van Steenbergen M" first="Mies J." last="Van Steenbergen">Mies J. Van Steenbergen</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Talsma, Herre" sort="Talsma, Herre" uniqKey="Talsma H" first="Herre" last="Talsma">Herre Talsma</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. 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Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Crommelin, Daan J A" sort="Crommelin, Daan J A" uniqKey="Crommelin D" first="Daan J. A." last="Crommelin">Daan J. A. Crommelin</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20602152</idno><idno type="pmc">2916118</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916118</idno><idno type="RBID">PMC:2916118</idno><idno type="doi">10.1007/s11095-010-0205-8</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000174</idno><idno type="wicri:Area/Pmc/Curation">000174</idno><idno type="wicri:Area/Pmc/Checkpoint">000391</idno><idno type="wicri:Area/Ncbi/Merge">000070</idno><idno type="wicri:Area/Ncbi/Curation">000070</idno><idno type="wicri:Area/Ncbi/Checkpoint">000070</idno><idno type="wicri:doubleKey">0724-8741:2010:Ghassemi A:hydrophilic:polyester:microspheres</idno><idno type="wicri:Area/Main/Merge">000D37</idno><idno type="wicri:Area/Main/Curation">000D35</idno><idno type="wicri:Area/Main/Exploration">000D35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA</title><author><name sortKey="Ghassemi, Amir H" sort="Ghassemi, Amir H" uniqKey="Ghassemi A" first="Amir H." last="Ghassemi">Amir H. Ghassemi</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Van Steenbergen, Mies J" sort="Van Steenbergen, Mies J" uniqKey="Van Steenbergen M" first="Mies J." last="Van Steenbergen">Mies J. Van Steenbergen</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Talsma, Herre" sort="Talsma, Herre" uniqKey="Talsma H" first="Herre" last="Talsma">Herre Talsma</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Van Nostrum, Cornelus F" sort="Van Nostrum, Cornelus F" uniqKey="Van Nostrum C" first="Cornelus F." last="Van Nostrum">Cornelus F. Van Nostrum</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Crommelin, Daan J A" sort="Crommelin, Daan J A" uniqKey="Crommelin D" first="Daan J. A." last="Crommelin">Daan J. A. Crommelin</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name><affiliation wicri:level="4"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author></analytic><series><title level="j">Pharmaceutical Research</title><idno type="ISSN">0724-8741</idno><idno type="eISSN">1573-904X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><sec><title>Purpose</title><p>To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA).</p></sec><sec><title>Methods</title><p>BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on <italic>in vitro</italic> release and degradation were studied. The integrity of the released BSA was studied by fluorescence spectroscopy and size exclusion chromatography (SEC).</p></sec><sec><title>Results</title><p>Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5–10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from ~20 days to 60 days when the HMG content decreased from 50% to 25%.</p></sec><sec><title>Conclusions</title><p>Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gupta, Pk" uniqKey="Gupta P">PK Gupta</name></author><author><name sortKey="Mehta, Rc" uniqKey="Mehta R">RC Mehta</name></author><author><name sortKey="Douglas, Rh" uniqKey="Douglas R">RH Douglas</name></author><author><name sortKey="Luca, Pp" uniqKey="Luca P">PP Luca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mehta, Rc" uniqKey="Mehta R">RC Mehta</name></author><author><name sortKey="Jeyanthi, R" uniqKey="Jeyanthi R">R Jeyanthi</name></author><author><name sortKey="Calls, S" uniqKey="Calls 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uniqKey="Koneberg R">R Koneberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vert, M" uniqKey="Vert M">M Vert</name></author><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author><author><name sortKey="Garreau, H" uniqKey="Garreau H">H Garreau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vert, M" uniqKey="Vert M">M Vert</name></author><author><name sortKey="Mauduit, J" uniqKey="Mauduit J">J Mauduit</name></author><author><name sortKey="Li, S" uniqKey="Li S">S Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schakenraad, Jm" uniqKey="Schakenraad J">JM Schakenraad</name></author><author><name sortKey="Hardonk, Mj" uniqKey="Hardonk M">MJ Hardonk</name></author><author><name sortKey="Feijen, J" uniqKey="Feijen J">J Feijen</name></author><author><name sortKey="Molenaar, I" uniqKey="Molenaar I">I Molenaar</name></author><author><name sortKey="Nieuwenhuis, P" uniqKey="Nieuwenhuis P">P Nieuwenhuis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Visscher, Ge" uniqKey="Visscher G">GE Visscher</name></author><author><name sortKey="Robison, Rl" uniqKey="Robison R">RL Robison</name></author><author><name sortKey="Maulding, Hv" uniqKey="Maulding H">HV Maulding</name></author><author><name sortKey="Fong, Jw" uniqKey="Fong J">JW Fong</name></author><author><name sortKey="Pearson, Je" uniqKey="Pearson J">JE Pearson</name></author><author><name sortKey="Argentieri, Gj" uniqKey="Argentieri G">GJ Argentieri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leemhuis, M" uniqKey="Leemhuis M">M Leemhuis</name></author><author><name sortKey="Kruijtzer, Jaw" uniqKey="Kruijtzer J">JAW Kruijtzer</name></author><author><name sortKey="Nostrum, Cf" uniqKey="Nostrum C">CF Nostrum</name></author><author><name sortKey="Hennink, We" uniqKey="Hennink W">WE Hennink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Sm" uniqKey="Li S">SM Li</name></author><author><name sortKey="Garreau, H" uniqKey="Garreau H">H Garreau</name></author><author><name sortKey="Vert, M" uniqKey="Vert M">M Vert</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Utrecht (province)</li></region><settlement><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Ghassemi, Amir H" sort="Ghassemi, Amir H" uniqKey="Ghassemi A" first="Amir H." last="Ghassemi">Amir H. Ghassemi</name></region><name sortKey="Crommelin, Daan J A" sort="Crommelin, Daan J A" uniqKey="Crommelin D" first="Daan J. A." last="Crommelin">Daan J. A. Crommelin</name><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name><name sortKey="Talsma, Herre" sort="Talsma, Herre" uniqKey="Talsma H" first="Herre" last="Talsma">Herre Talsma</name><name sortKey="Van Nostrum, Cornelus F" sort="Van Nostrum, Cornelus F" uniqKey="Van Nostrum C" first="Cornelus F." last="Van Nostrum">Cornelus F. Van Nostrum</name><name sortKey="Van Steenbergen, Mies J" sort="Van Steenbergen, Mies J" uniqKey="Van Steenbergen M" first="Mies J." last="Van Steenbergen">Mies J. Van Steenbergen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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